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Introduction to Molecular Methods for Algae Research

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This course introduces you to a variety of molecular methods for algae research including DNA extraction, clean-up, DNA sequencing, gene expression and bioinformatic analysis.

Molecular biol 6This course introduces you to a variety of molecular methods including DNA extraction, clean-up, DNA sequencing, gene expression and bioinformatic analysis. 

Focusing on laboratory and bioinformatics skills relevant to algal research, this two-day course introduces up-to-date molecular approaches to investigate the biology and ecology of algae in coastal and ocean habitats from the tropics to the poles.  The course addresses practical information and modifications to standard molecular protocols required to achieve success with algal material.

An ideal follow-on to the Algaculture for Biotechnology course which directly precedes it. The molecular methods course comprises a mixture of lectures and hands-on sessions (lab and IT based).  The course is ideally suited to PhD students, laboratory workers and researchers wanting to start applying molecular methods in their algal research.

What you’ll learn on this predominantly hands-on course:


Day 1 (29th October)Algae Research

  • Algal DNA extraction
  • Lecture: Principals of nucleic acid extraction
  • Laboratory: DNA extraction
  • The polymerase chain reaction (PCR)
  • Lecture: Principals of the PCR
  • Lecture: Principals of PCR Amplicon cloning and DNA sequencing
  • Laboratory: PCR

 

Day 2 (30th October)

  • Lecture: Principals of quantitative PCR (qPCR) and gene expression
  • Laboratory: PCR
  • Bioinformatics
  • DNA sequence assembly and analysis
  • Introduction to phylogenetic analysis

 

Specific learning outcomes

  • Comprehensive introduction to theory “how, what and when” of DNA extraction with a specific focus on the unique challenges of working with algae, and consolidated with 4.5 hr hands-on laboratory practice.
  • Comprehensive introduction to the theory and practice of the PCR and protocols for PCR cloning, and the power of quantitative PCR for relative and absolute quantitation of genes or gene expression.
  • Comprehensive introduction to bioinformatics emphasising available software and databases, coupled with a 3 hour hands-on exercise assembling and manipulating algal DNA sequence data and phylogenetic analysis.


Your tutors are all highly experienced scientists at the Scottish Association for Marine Science (SAMS) and/or CCAP (the NERC Culture Collection of Algae and Protozoa; the most diverse collection of its kind in the world, with 3000 strains of marine and freshwater algae, protists and seaweeds).  The course is led by Dr David Green who has extensive experience in molecular microbial ecology and bioinformatic analysis.


Places on this course are strictly limited to provide you with hands-on experience and plenty of time with the scientists.  The course is ideally suited to PhD students, laboratory workers and researchers wanting to start applying molecular methods in their algal research.  It is recognised by the Institute of Marine Engineering, Science and Technology (IMarEST).  Attendance counts towards your Continuing Professional Development for both the IMarEST and The Science Council’s accolade of Chartered Scientist (CSci).
 

Price: £540 (VAT exempt) including all course materials, laboratory equipment and lunch on both days.  You may want to arrange overnight accommodation in Oban if required.  (This is not included in the course fee.)

 

Please apply for your Molecular Methods for Algae Research Course online, email SAMSCourses@sams.ac.uk or call 01631 559000

 

SPECIAL OFFER:

This course is preceded by an Algaculture for Biotechnology Course, which runs on the 27th and 28th of October.  You may wish to attend both courses whilst you’re visiting SAMS, in which case, a discount of £70 is available.  Please book online using this link to ensure you receive the discount or contact us for further details.

FUNDING:

PHYCONET logo 2014 jpg.jpgA limited amount of funding has been offered by PHYCONET (a BBSRC NIBB).  As a result, we can provide a subsidy of £100 per person to the first ten people who apply for either this course, or the Algaculture for Biotechnology Course.  (This is a subsidy of £100 per person and not per course.)  To take advantage of this funding and ensure you receive the discount, you will need to book manually with us.  Please email SAMSCourses@sams.ac.uk or call 01631 559000 for further details.

 

Student members of the British Phycological Society (BPS) may be eligible for funding towards SAMS Algal short courses.  Details of the BPS competitive funding scheme can be found via http://www.brphycsoc.org/funding.lasso.  There are 4 closing dates per year to apply (you must be a student); 1st March, 1st June, 1st Oct and 1st Dec and you must have been a BPS member for 3 months ahead of your application.

 

You may also be interested in our new Masters Degree in Algal Biotechnology. This new 12 month Masters by Research (MRes) programme will provide you with the specialist knowledge and skills required for a career in this exciting growth sector.

 

Instructors:


David GreenDr David Green is a leading international researcher in algal-bacterial interactions.  He is active in commercial research of marine bacteria that produce bioemulsifiers and ethanol fermentation from seaweed. He is actively involved with marine microbial sulphur and iron biogeochemistry.  

 

 


Dr Claire Gachon is interested in all aspects of algal host-pathogen interactions.  Her current research delves into the Genomics of Marine Algae and their Pathogens and she recently published a joint paper questioning whether brown algae might be capable of adaptive immunity.  Claire has recently been invited to speak in Korea, Japan and Bordeaux.  She sits on the Steering committee of NERC’s National Biomolecular Analysis Facility and is also an Evaluator for Marie Curie Actions.


Dr Mark Hart is interested in a broad range of molecular microbial questions. He currently works on measuring gene expression in marine sedimenting material linked to methane production, and in applying molecular methods for the detection of toxic marine algae.

 

What Is The Department of Microbial and Molecular Biology?


The Microbial and Molecular Biology Department spans biochemistry, ecology, molecular biology and mathematical modelling.  Their activities focus predominantly on the micro-organisms which drive marine ecosystems and play a major role in global climate and life-support systems.

A wide range of fundamental and applied research projects are currently underway in the department, including biofuels, harmful algal blooms, marine macro-algae (seaweeds) and polar microbial ecology.  These projects employ the molecular biology that will be taught on this course.

Please apply for your Molecular Methods for Algae Research Course online, email SAMSCourses@sams.ac.uk or call 01631 559000

SPECIAL OFFER:

This course is preceded by an Algaculture for Biotechnology Course, which runs on the 27th and 28th of October.  You may wish to attend both courses whilst you’re visiting SAMS, in which case, a discount of £70 is available.  Please book online using this link to ensure you receive the discount or contact us for further details.

 

Student members of the British Phycological Society (BPS) may be eligible for funding towards SAMS Algal short courses.  Details of the BPS competitive funding scheme can be found via http://www.brphycsoc.org/funding.lasso.  There are 4 closing dates per year to apply (you must be a student); 1st March, 1st June, 1st Oct and 1st Dec and you must have been a BPS member for 3 months ahead of your application.

 

You may also be interested in our new Masters Degree in Algal Biotechnology. This new 12 month Masters by Research (MRes) programme will provide you with the specialist knowledge and skills required for a career in this exciting growth sector.

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